Cereal Chem 38:103 - 108.  |  VIEW ARTICLE

Histochemical Characterization of Wheat and Wheat Products. I. Histochemical Demonstration of Germ and Aleurone Using Acridine Orange.

Y. Pomeranz and J. A. Shellenberger. Copyright 1961 by the American Association of Cereal Chemists, Inc. 

Polychromatic fluorescent properties of acridine orange solutions were used to characterize wheat tissues and milled wheat products. A water solution of the dye can be used for multicolor staining of wheat tissues. Coloration varies with protein content.A saturated xylol (xylene) solution of the base forms a brilliant fluorescence with the lipid material of either the aleurone or the germ so it can be used to distinguish these two wheat tissues in milled products.