Cereal Chem 38:109 - 112.  |  VIEW ARTICLE

Histochemical Characterization of Wheat and Wheat Products. II. Mapping of Protein Distribution in the Wheat Kernel.

Y. Pomeranz and J. A. Shellenberger. Copyright 1961 by the American Association of Cereal Chemists, Inc. 

Topographical distribution of protein in wheat was followed on sections cut with a freezing microtome by means of the preferential adsorption of Xylidine Ponceau. The outer endosperm portions showed a higher dye-binding capacity than the central portions and the cheeks. Lowest protein concentration was in the pericarp; highest, in the aleurone layer and embryo.