Cereal Chem 45:1 - 12.  |  VIEW ARTICLE
Physicochemical Properties of Protein of Developing and Mature Rice Grain.

E. P. Palmiano, A. M. Almazan, and B. O. Juliano. Copyright 1968 by the American Association of Cereal Chemists, Inc. 

Analyses were carried out on acetone powders of the whole grain of Oryza sativa L. (variety IR8) during development, from a few days after flowering to maturity. Nonprotein nitrogen decreased in concentration, but the amount per grain remained relatively constant. The protein fractions per grain increased during the first 2 weeks after flowering, but only glutelin and prolamin continued to increase in amount throughout development. Albumin-globulin per grain and the concentration of urea-sodium chloride extract of the acetone powder decreased during the later stages of development. Gel filtration of the urea-salt extract on Sephadex G-100 gave three fractions corresponding to molecular weights of greater than 2, 0.8, and 0.3 x 10(5). Amino acid analysis also showed the 4-day sample to have a higher lysine content than did either the 14-day sample or the mature grain. These data were interpreted on the basis of analysis of preparations of protein fractions of brown rice.