Cereal Chem 46:177 - 180. | VIEW
ARTICLE
Thin-Layer Chromatography of Malto-Oligosaccharides.
P. Haytko, R. Burns, and C. E. Weill. Copyright 1969 by the American Association of Cereal Chemists, Inc.
Thin-layer chromatographic experiments have been conducted on the homologous series of malto- oligosaccharides with various proportions of two different solvent systems (t-butyl alcohol:water and n- butyl alcohol:pyridine:water) on two support media, Silica Gel G and Kieselguhr G. A plot of log10 Rf/(1 - Rf) against the number of monomer units in the oligosaccharide yields a series of straight lines for the various proportions of each of the solvent systems. The height of the common intercept of these lines is dependent more on the nature of the support medium than on the nature of the solvent systems. Suggestions are made to help govern the selection of the proper support medium and solvent mixture.