Cereal Chem 47:54 - 63.  |  VIEW ARTICLE
An Endo-Beta-1, 4-Glucan Hydrolase from Germinated Barley.

D. J. Moffa and W. W. Luchsinger. Copyright 1970 by the American Association of Cereal Chemists, Inc. 

An endo-beta-1, 4-glucan hydrolase present in sodium chloride extracts of germinated barley was purified over 100-fold by dialysis, chromatography on DEAE-cellulose, redialysis, and rechromatography on DEAE-cellulose. Disc electrophoresis revealed one major and eight minor proteins. The activity of the preparation on carboxymethyl cellulose had the following characteristics: a pH optimum of 5.0, stimulation by sodium chloride with maximum stimulation at 0.08M, stable at 40 C., and rapidly inactivated at 60 C., inhibited by celloboise, glucono-1,5-lactone, and N-bromosuccinimide, not inhibited by phenylmercuric nitrate and several other compounds. The preparation also hydrolyzed barley beta-D-glucan but showed no action on insolubvle cellulose or laminarin.