Cereal Chem 49:453 - 457.  |  VIEW ARTICLE
The Use of Reduced Beta-Limit Dextrin as Substrate in an Automated Amylase Assay.

J. E. Kruger and B. Marchylo. Copyright 1972 by the American Association of Cereal Chemists, Inc. 

A sensitive automated method for determining alpha-amylase has been developed, using sodium borohydride-reduced beta-limit dextrin as substrate and employing a Technicon AutoAnalyzer. Hydrolysis products from the reaction between reduced beta-limit dextrin and enzyme were reacted with potassium ferricyanide and the decrease in absorbance measured at 420 nm. The method was illustrated with amylases from fungal, bacterial, and plant sources. Beta-amylase increased the sensitivity of the assay and must be added in excess for alpha-amylase sources containing this enzyme.