Cereal Chem 50:101 - 105.  |  VIEW ARTICLE
Chemical Methods for Available Lysine.

J. W. Finley and M. Friedman. Copyright 1973 by the American Association of Cereal Chemists, Inc. 

Current procedures for available lysine are based on modification of the epsilon-amino groups with 1- fluoro-2,4-dinitrobenzene or 2,4,6,-trinitrobenzenesulfonic acid. The proposed new method is based on modification of the lysine epsilon-amino group with activated vinyl compounds, such as methyl acrylate, followed by acid hydrolysis and ion-exchange chromatography on an amino acid analyzer. The methyl acrylate procedure for available lysine complements currently used methods and may have advantages in some applications.