Cereal Chem 50:210 - 214.  |  VIEW ARTICLE
Sucrose Determination by a Modified Anthrone Method. Application with Sweetened Wheat-Soy Blend and Corn-Soy-Milk.

J. W. Finley and D. A. Fellers. Copyright 1973 by the American Association of Cereal Chemists, Inc. 

The colorimetric method developed is based on extraction of sugars with 80% ethanol, destruction of interfering reducing sugars with Fehling solution, and reaction of remaining sucrose with anthrone in sulfuric acid at 40 C. Absorbance of the sucrose-anthrone complex is read at 610 nm. and compared with the absorbance of a similarly treated standard sucrose solution. An automated method using a Technicon AutoAnalyzer is also described. Application of the method to determine added sucrose in sweetened wheat- soy blend and sweetened corn-soy-milk for quality assurance and regulatory purposes is described.