Cereal Chem 51:316 - 322.  |  VIEW ARTICLE
The Protein Fractions in Chemically Induced High-Protein Wheat Seed.

V. D. Strbac, G. S. Ayers, and S. K. Ries. Copyright 1974 by the American Association of Cereal Chemists, Inc. 

The protein content of Arthur winter wheat grain was increased by a foliar application of 70 g. per ha. terbacil (3-tert-butyl-5-chloro-6-methyluracil). The protein content of Inia spring wheat grain was increased by applications of both 250 g. per ha. simazine (2-chloro-4,6-bis-ethylamino s-triazine) and supplemental nitrogen. The proteins from the seed were separated into albumins, globulins, gliadins, and glutenins by appropriate solvent extractions. The higher protein seed, based on percent total nitrogen, obtained either by chemical or nitrogen applications, contained more nitrogen as gliadins than seed from the control plots for both varieties, whereas simazine reduced the percent of total meal nitrogen incorporated into the albumin fraction of Inia spring wheat. The gliadins from high- and low-protein seed differed electrophoretically.