Cereal Chem 51:586 - 591.  |  VIEW ARTICLE
Rapid Method for Determining the Zein Content of Whole Maize Seed or Isolated Endosperm.

A. Dalby. Copyright 1974 by the American Association of Cereal Chemists, Inc. 

Ground maize seed is extracted with 70% ethanol at 60 C. for 90 min. and an aliquot transferred to a filter paper disc. Discs are washed in bulk through the series: saline, water, ether, acetone, ether, to remove non- zein impurities, and the zein nitrogen is determined by micro-Kjeldahl analysis on the air-dried discs. Reproducibility is good and the technique permits large numbers of samples to be processed quickly and simply.