Cereal Chem 51:605 - 609.  |  VIEW ARTICLE
High-Resolution Liquid Chromatography of Vitamin E Isomers.

J. F. Cavins and G. E. Inglett. Copyright 1974 by the American Association of Cereal Chemists, Inc. 

High-resolution liquid adsorption chromatography on a 2-m. Corasil II column was found to provide optimum separation of the eight naturally occurring vitamin E isomers. Columns were eluted by a single solvent mixture at a flow rate of 1 ml. per min. and separation was achieved in approximately 1.75 hr. The flow rate or the solvent polarity may be increased to speed elution when complete separation is not necessary. The procedure has been used for preparative purposes.