Cereal Chem 53:705 - 710.  |  VIEW ARTICLE
Studies on Corn Proteins. X. Polypeptide Molecular-Weight Distribution in Landry-Moureaux Fractions of Normal and Mutant Endosperms.

P. S. Misra, E. T. Mertz, and D. V. Glover. Copyright 1976 by the American Association of Cereal Chemists, Inc. 

The endosperm proteins of normal corn inbred Oh 43 and mutants o2, fl2, fl2o2, bt2, and bt2o2, as well as normal corn inbred W22 and its mutant o7, were separated into fractions by the Landry-Moureaux method. Based on molecular weights determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, fraction I (saline-soluble) had major polypeptides with average molecular weights 58,000, 24,500, 22,000, and 13,400 daltons. Fraction II (zein), with the exception of bt2o2, contained major polypeptides with average molecular weights of 25,000 and 21,800 daltons. Fraction III (zein-like) had major polypeptides with average molecular weights of 26,000, 23,000, and 18,000 daltons, and fraction IV (glutelin-like) had major polypeptides with average molecular weights of 61,000, 58,000, 25,700, and 19,000 daltons. Fraction V (true glutelin) polypeptides did not separate clearly on the gel. The 25,000 dalton component of fraction II in o2 and fl2o2 is reduced below that in normal, fl2, and o7. The 44,000 dalton compo nent of fraction II is a unique component of fl2 and fl2o2, as is the 14,000 dalton component of fraction III in o2 and o7.