Cereal Chem 55:967 - 972.  |  VIEW ARTICLE
Determination of Coumestrol in Soybeans by High-Performance Liquid and Thin-Layer Chromatography.

G. L. Lookhart, B. L. Jones, and K. F. Finney. Copyright 1978 by the American Association of Cereal Chemists, Inc. 

Coumestrol, an estrogenic compound found in many forage plants, was determined quantitatively (0.05-30 ppm) in soybean (Glycine max L. Merrill) extracts by high-performance liquid chromatography (HPLC). The extraction procedure required 3 hr and the HPLC analysis 12 min. A quantitative estimate of coumestrol in the extract was made by thin-layer chomatography in 25 min with a sensitivity limit of 10 ppm and 2 min for a sensitivity of more than 50 ppm.