Cereal Chem 56:446 - 451.  |  VIEW ARTICLE
Endosperm Structure of Barley Isogenic Lines.

B. D. Bechtel and Y. Pomeranz. Copyright 1979 by the American Association of Cereal Chemists, Inc. 

Light microscopy (LM) and tranmission electron microscopy (TEM) were used to compare two pairs of isogenic lines of barley: Hiproly (high protein-high lysine, CI 3947) vs high protein (CI 4362) and normal starch (JGM 57) vs waxy starch (JGM 58). Hiproly and high protein (CI 4362) were compared with a widely grown cultivar, Firlbecks. The subaleurone regions of both Hiproly and high protein (CI 4362) were enlarged, but that of the high protein was irregular in thickness. Two major types of proteins were detectable by both LM and TEM. One type stained magenta with toluidine bule O and basic fuchsin and appeared homogeneous and electron-lucent in the TEM. The other type stained purple in the LM and was heterogeneous and electron-dense in the TEM. Hiproly, CI 4362, and Firlbecks differed in the amounts and shapes of the two protein types. In LM, the starch granules in the waxy starch line were distorted and irregular in shape. In TEM, the waxy starch granules appeared mottled and the normal starch granules were uniformly stained.