Cereal Chem 56:100 - 104.  |  VIEW ARTICLE
Composition of Sesame Seed Protein Components and Purification of the Main Globulin.

K. Okubo, N. Nishimura, and K. Shibasaki. Copyright 1979 by the American Association of Cereal Chemists, Inc. 

Phosphate buffer (pH 7.66) containing 0.02M2-mercaptoethanol and 10% sodium chloride was a satisfactory medium for the extraction of proteins from defatted sesame seed meal. The extracted whole proteins contained more than 85% of the 13S globulin components. The globulin fraction was precipitated by dialysis of the extract against water. After purification by gel filtration, the 13S globulin was homogeneous when examined by ultracentrifugation, disc gel electrophoresis, and double gel immunodiffusion. Ultracentrifuge and slab gel electrophoresis analyses in the presence of urea showed that the 13S globulin has a subunit structure composed of acidic and basic subunits.