Cereal Chem 56:267 - 271.  |  VIEW ARTICLE
Characteristics of Phytase and Its Relationship to Acid Phosphatase and Certain Minerals in Triticale.

B. Singh and H. G. Sedeh. Copyright 1979 by the American Association of Cereal Chemists, Inc. 

Some properties of triticale phytase were studied using both crude extract in sodium acetate buffer and partially purified extract. The optimum pH of the enzyme was 5.4 at 45 C. The Michaelis-Meten constant (km) of the partially purified extract was 0.22 x 10(-3) M. The enzyme was slightly activated by magnesium and manganese salts. The activity was inhibited by p-chloromercuribenzoate and the salts of iron, copper, silver, nickel, and cobalt. With few exceptions, triticale bran had higher phytase and acid phosphatase activity than flour or whole grain, as well as higher amounts of phytic acid, calcium, magnesium, zinc, and iron. Simple correlation coefficients, determined in all possible combinations, indicated no significant correlations between phytase, phosphatase, phytic acid, calcium, magnesium, zinc, and iron. There were however, significant correlations between calcium, zinc, and phosphatase; magnesium, zinc, and phytic acid; and iron and phosphatase.