Cereal Chem 57:411 - 415.  |  VIEW ARTICLE
Evaluation of a Nephelometric Method for Determining Cereal Alpha-Amylase.

B. T. O'Connell, G. L. Rubenthaler, and N. L. Murbach. Copyright 1980 by the American Association of Cereal Chemists, Inc. 

A nephelometric assay adapted from a clinical method was evaluated for suitability in determining alpha- amylase levels in sprouted soft white wheat. In the manual mode of machine operation, the velocity of decline in light scattering was directly proportional to the amount of enzyme assayed. The accurate limits of the assay performed in the automated mode were defined as 0-720 machine units of activity, with a commercial Beta-limit dextrin as substrate. This substrate was more reactive than amylopectin and less subject to Beta-amylase interference. The automated assay yielded data that correlated highly with falling number results.