Cereal Chem 58:207 - 210.  |  VIEW ARTICLE
Isolation of Trypsin Inhibitors from Rye, Triticale, and Wheat Samples.

C.-R. Chang and C. C. Tsen. Copyright 1981 by the American Association of Cereal Chemists, Inc. 

Trypsin inhibitors (TI) of rye, triticale, hard red winter wheat, and durum wheat were isolated by extraction, affinity chromatography, CM-cellulose ion-exchange chromatography, and gel filtration chromatography. Affinity chromatography produced a relatively high yield (82 and 83%, respectively) for rye and triticale TI but a poor yield (37 and 29%, respectively) for hard red winter wheat and durum wheats TI. The specific activity of the inhibitors was increased 100-200 times by this step. Affinity chromatography resolved predominantly one rye TI (mol wt 17,000), one triticale TI (mol wt 19,500), two hard red winter wheat TI (mol wt 36,800 and 21,000), and two durum wheat TI (mol wt 38,500 and 22,500). CM-cellulose ion- exchange and gel filtration steps further purified the inhibitors to increase their specific activities.