Cereal Chem 59:516 - 519.  |  VIEW ARTICLE
Near-Infrared Reflectance for Analysis of Cottonseed for Gossypol.

G. S. Birth and H. H. Ramey, Jr. Copyright 1982 by the American Association of Cereal Chemists, Inc. 

Gossypol, a toxic yellow pigment in glanded cottonseed, is a major obstacle to the use of cottonseed for human consumption. Near-infrared reflectance spectroscopy (NIR) was investigated as a means of rapidly determining the gossypol content of cottonseed. Cottonseed samples were delinted and ground. Reflectance spectra from 1.0 to 2.38 micrometers were recorded followed by derivative data processing. The reflectance data and data obtained from chemical analysis of the cottonseed samples were used in a stepwise multiple regression analysis to develop an equation to express the gossypol content of cottonseed based on reflectance spectra alone. The results, using 30 samples, yielded a multiple correlation coefficient of 0.987 for a three-term equation, and a standard error of 0.074. Comparable results were obtained when the analysis was repeated with an independent group of samples.