Cereal Chem 59:108 - 112.  |  VIEW ARTICLE
Results of Collaborative Testing Using a Simplified, Rapid Colorimetric Alpha-Amylase Assay for Evaluation of Sprouted Wheat.

P. R. Mathewson, C. H. Fahrenholz, G. D. Booth, Y. Pomeranz, and B. S. Miller. Copyright 1982 by the American Association of Cereal Chemists, Inc. 

The procedure and equipment required for a simplified colorimetric alpha-amylase assay are described, as are the results of two collaborative studies utilizing this method for the evaluation of sprout damage in wheat. Collaborative testing, using wheat having from 0 to 8% sprouted kernels, demonstrated that the different levels of sprout damage were distinguishable, despite statistically significant sampling error for whole wheat samples. In addition, collaborators within a laboratory were able to achieve reproducible results on their own equipment. The use of a standardized, commercially available instrument designed to perform this test should provide a reliable, rapid procedure for the evaluation of sprout damage.