Cereal Chem 60:192 - 194.  |  VIEW ARTICLE
Rapid Methods for Estimating Protein and Lysine in Sorghum (Sorghum bicolor (L.) Moench).

R. Jambunathan, N. S. Rao, and S. Gurtu. Copyright 1983 by the American Association of Cereal Chemists, Inc. 

Sorghum (Sorghum bicolor (L.) Moench) samples were analyzed for protein by the Technicon auto analyzer (TAA) method, and the results were compared with the micro-Kjeldahl method. The TAA method was highly and significantly correlated (r = 0.997**) with the micro-Kjeldahl method, and the results were found to be highly reliable for routine screening of samples. Lysine was estimated by the rapid dye-binding capacity (DBC) procedure and was determined using an amino-acid analyzer. The results of the DBC procedure, when expressed as a ratio with respect to the protein content, were highly and positively correlated (r = 0.933**) with the amino-acid analyzer values. The rapid procedures of TAA and DBC were observed to give reliable estimates of protein and lysine content, respectively, and are recommended when large numbers of sorghum samples are to be analyzed.