Cereal Chem 61:344 - 349.  |  VIEW ARTICLE
Modification of the Anthrone, Carbazole, and Orcinol Reactions for Quantitation of Monosaccharides.

W. H. Kunerth and V. L. Youngs. Copyright 1984 by the American Association of Cereal Chemists, Inc. 

The anthrone, carbazole, and orcinol reactions used for quantitation of hexoses, uronic acids, and pentoses, respectively, in Southgate's method for the analysis of unavailable carbohydrates (fiber) were modified to improve specificity by measuring the absorbance at two wavelenghts and by using multiple linear regression techniques to correct for remaining interferences. The results of the anthrone reaction were used to correct for hexose interferences in the uronic acid and pentose determinations, and the results of the carbazole reaction were used to correct for uronic acid interferences in the pentose determination.