Cereal Chem 61:544 - 548.  |  VIEW ARTICLE
Separation of Wheat Gliadins by Preparative Reversed-Phase High-Performance Liquid Chromatography.

F. R. Huebner and J. A. Bietz. Copyright 1984 by the American Association of Cereal Chemists, Inc. 

A preparative method is described for separating wheat gliadins in significant quantities by using reversed- phase high-performance liquid chromatography. The method has advantages over previous methods in simplicity and speed. It separates proteins by hydrophobicity rather than by size or ionic strength. This, in conjunction with separations by molecular size and by ion-exchange chromatography, enabled further separation of four of those fractions into at least 15 subfractions. The separated fractions were also analyzed by aluminum lactate gel electrophoresis and by isoelectric focusing, which showed that each of the above fractions still contained more than one component.