Cereal Chem 63:497-500   |  VIEW ARTICLE
Comparison of Polyacrylamide Gel Electrophoresis and High-Performance Liquid Chromatography Analyses of Gliadin Polymorphism in the Wheat Cultivar Newton.

G. L. Lookhart, L. D. Albers, and J. A. Bietz. Copyright 1986 by the American Association of Cereal Chemists, Inc. 

Polyacrylamide gel electrophoresis (PAGE) and reversed-phase high-performance liquid chromatography (HPLC) are highly effective and complementary techniques for differentiation of wheat genotypes through gliadin analysis. To compare diectly their relative abilities to detect kernel-to-kernel variation within a variety, 100 individual kernels of the wheat variety Newton were cut in two, and gliadins were extracted from each half and analyzed by the two methods. Two major genotypes were observed in a ratio of 42:58. In addition, there was a perfect correlation between the methods for genotype identification of each individual kernel. The PAGE patterns of the isolated HPLC peaks that were different for the two genotypes were identical to the band patterns that were characteristic of each genotype, demonstrating that the same proteins were responsible for the major differences, which were readily apparent by either method.