Cereal Chem 66:193-196   |  VIEW ARTICLE

Study of Proteins Extracted from the Surface of Wheat Starch Granules with Sodium Dodecyl Sulfate.

M. Seguchi and Y. Yamada. Copyright 1989 by the American Association of Cereal Chemists, Inc. 

Wheat prime starch granule surface proteins were extracted with 1% sodium dodecyl sulfate (SDS) solution containing 1% 2-mercaptoethanol at room temperature without swelling and gelatinization of the starch granules. The extracted prime wheat starch granules showed no staining with amido black 10B, indicating no stainable proteins remained on the surface of the starch granules. SDS gel electrophoresis of the extracts stained with Coomassie brilliant blue R-250 and silver stain showed the pattern of the extracted surface protein bands. These protein bands were also subjected to periodic acid-Schiff and Sudan black staining; however, neither glycoproteins or lipoproteins were observed. A low molecular weight band was observed in the gel and identified as lysophosphatidyl choline after electro-extraction following thin-layer chromatography. After five successive extraction treatments of the same mixture, changes in the prime starch granules became apparent under observation with phase-contrast microscopy.