Cereal Chem 67:129-135   |  VIEW ARTICLE

Rice Cultivar Identification by High-Performance Liquid Chromatography of Endosperm Proteins.

F. R. Huebner, J. A. Bietz, B. D. Webb, and B. O. Juliano. Copyright 1990 by the American Association of Cereal Chemists, Inc. 

Rice varietal identification is important for breeding new varieties and for marketing varieties for specific uses. Previous identification methods were not satisfactory. We therefore analyzed rice prolamins (soluble in high concentrations of alcohol), salt-soluble proteins, and glutelins by reversed-phase high-performance liquid chromatography (RP-HPLC) to find a better method. Using improved extraction and RP-HPLC conditions, glutelins, rice's major storage proteins, best differentiate varieties. RP-HPLC also reveals that amounts of protein classes differ among varieties.