Cereal Chem 68:396-400   |  VIEW ARTICLE

Effect of Solvent Extraction, Environment, and Genetic Background on Differentiating Rice by Reversed-Phase High-Performance Liquid Chromatography.

G. L. Lookhart, B. O. Juliano, and B. D. Webb. Copyright 1991 by the American Association of Cereal Chemists, Inc. 

The effects of solvent (protein fraction) and location of growth (environmental) were studied on milled U.S. and non-U.S. rices extracted by four separate solvent systems: 60% 1-propanol, 70% ethanol, 60% 1- propanol plus 1% dithiothreitol, and 5% acetic acid. The high-performance liquid chromatography (HPLC) patterns of the alcohol extracts of five U.S. varieties (including short-, medium-, and long-grain rices) grown in five states showed minor but consistent differences depending on location of growth. HPLC patterns of 5% acetic acid extracts of the same varieties differed more than did the patterns of the alcohol extracts among locations for the U.S. varieties. Therefore, identifying varieties from different environments requires the use of alcohol extracts. HPLC patterns for the Philippine rice IR36 also showed environmental effects of extraction solvent similar to those for U.S. rices. Prolamin patterns of international rices of different genetic backgrounds differed. In 10 lines from the International Rice Research Institute, the prolamin HPLC patterns of progeny usually were similar to those of one parent.