Cereal Chem 68:200-204   |  VIEW ARTICLE

Immunological Distinction Between x-Type and y-Type High Molecular Weight Glutenin Subunits.

A. Curioni, A. D. B. Peruffo, G. Pressi, and N. E. Pogna. Copyright 1991 by the American Association of Cereal Chemists, Inc. 

Polyclonal antisera were raised against four high molecular weight glutenin subunits purified by sodium dodecyl sulfate electroendosmotic preparative electrophoresis from the wheat cultivar Clara 2. Immuno- blotting with total protein extracts and gliadins from several wheat cultivars revealed that the antisera to x- subunits 1, 5, and 7 bound only to high molecular weight glutenin subunits, whereas the antiserum to y- subunit 12 also reacted with alpha-, beta- and gamma-gliadins. The antisera to subunits 1 and 5 reacted more strongly with x-subunits than with y-subunits, and the antiserum to subunit 12 showed more reaction with y-subunits. Comparison of amino acid sequences suggests that some antigenic determinants typical of the y-type and x-type subunits lie in the N-terminus and in the central repetitive domain of the protein, respectively.