Cereal Chem 68:312-316   |  VIEW ARTICLE

Identification of Wheat Lines Containing the 1BL/1RS Translocation by High-Performance Liquid Chromatography.

G. L. Lookhart, R. Graybosch, J. Peterson, and A. Lukaszewski. Copyright 1991 by the American Association of Cereal Chemists, Inc. 

Analysis by high-performance liquid chromatography (HPLC) of 70% ethanol-solubilized grain proteins was used to identify 1BL/1RS in a sample of 63 experimental wheat lines and six released cultivars. The veracity of this technique was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separation of reduced grain proteins, Southern blotting of genomic DNA followed by hybridization with a rye-specific DNA probe, and direct karyotyping of chromosomes of each tested line. Simultaneous application of multiple techniques for identifying 1RS in wheat genetic backgrounds is suggested as a way to isolate new translocation lines that lack undesirable gene products. Of the methods studied, only HPLC and SDS-PAGE can be used to determine the presence of lines carrying the rye translocation in flours; the other techniques require viable grain samples. Presently, only direct karyotypic analyses can differentiate a 1BL/1RS translocation from a 1AL/1RS or a 1DL/1RS translocation.