Cereal Chem. 70:458-463   |  VIEW ARTICLE

Detection of Wheat-Rye Chromosomal Translocations Using an Antisecalin Monoclonal Antibody.

R. Graybosch, Y. W. Seo, and C. J. Peterson. Copyright 1993 by the American Association of Cereal Chemists, Inc. 

Hybridoma lines derived from mice immunized with a secalin preparation from the rye (Secale cereale L.) cultivar Rymin were analyzed for differential reactions with rye secalins and wheat (Triticum aestivum L.) gliadins. A single cell line, secreting a monoclonal antibody (MAB) with high affinity for rye secalin but comparatively low cross-reactivity with gliadin, was identified. After electrophoretic separation of unreduced proteins, immunoblotting experiments demonstrated specific binding to omega-secalins encoded by genes located on rye chromosome 1RS. When used in indirect enzyme-linked immunosorbent assay, the antisecalin monoclonal antibody allowed the discrimination of rye from wheat, the estimation of the percentage of rye in mechanical mixtures of wheat and rye, and the identification of wheat lines carrying wheat-rye chromsomal translocations involving 1RS.