Cereal Chem 72:252-254  |  VIEW ARTICLE

Assessment of Enzyme-Linked Immunoassay of Rye Secalins as a Tool in the Prediction of 1RS Wheat Quality.

Y. W. Seo, R. A. Graybosch, C. J. Peterson, and D. R. Shelton. Copyright 1995 by the American Association of Cereal Chemists, Inc. 

Incorporation of rye chromatin into the wheat genome often has resulted in a subsequent loss of dough quality. A monoclonal antibody, specific for secalins (rye endosperm storage proteins) was tested as a means of consistently identifying wheats carrying rye chromosome arm 1RS and predicting their end-use quality. Mean absorbances (A) in enzyme-linked immunosorbent assays (ELISA) of 1AL/1RS or 1BL/1RS wheats from 16 production environments were significantly higher than those observed with non-1RS wheats. Significantly higher absorbances also were observed in 1AL/1RS wheats as compared to 1BL/1RS wheats. Among 1AL/1RS wheats, however, ELISA absorbances were associated with increased total flour protein content, a factor contributing to higher wheat quality scores. Among 1BL/1RS wheats, where absorbances were independent of total protein content, significant negative correlations with quality were detected. ELISA using this antisecalin monoclonal antibody has more utility as a means of identifying 1RS wheats in breeding programs than as a tool for predicting flour quality in the grain trade.