Cereal Chem. 73 (6):729-731  |  VIEW ARTICLE

Analytical Techniques and Instrumentation

Furosine Determination in Baby Cereals by Ion-Pair Reversed-Phase Liquid Chromatography.

E. Guerra-Hernandez (1) and N. Corzo (2). (1) Departamento de Nutrición y Bromatología. Facultad de Farmacia. Universidad de Granada. Campus Universitario de Cartuja. 18012, Granada, Spain. Corresponding author. E-mail: <ejguerra@platon.urg.es> (2) Instituto de Fermentaciones Industriales (C.S.I.C.) Juan de la Cierva, 3. 28006, Madrid, Spain. Accepted August 12, 1996. Copyright 1996 by the American Association of Cereal Chemists, Inc. 

A simple procedure for the determination of epsilon-N-2-furoylmethyl-L-lysine (furosine) in baby cereals is reported. Furosine was quantified in acid hydrolysates by isocratic ion-pair reversed-phase liquid chromatography using a C(18) column and UV detection at 280 nm. The detection limit was 1.42 pmol. Furosine formation by sample hydrolysis with hydrochloric acid increased with acid concentration to 10.6M. The content of furosine found in baby cereals ranged from 293 to 3,180 mg/100 g of protein.