Cereal Chem. 73 (3):388-391  |  VIEW ARTICLE

Mycotoxins

Distribution of Deoxynivalenol and Zearalenone in Milled Fractions of Wheat (1).

D. M. Trigo-Stockli (2), C. W. Deyoe (2), R. F. Satumbaga (2,3), and J. R. Pedersen (2). (1) Contribution 95-535-J. Kansas Agricultural Experiment Station, Kansas State University, Manhattan 66506. Presented at the AACC 79th Annual Meeting, Nashville, TN, October 1994. (2) Department of Grain Science and Industry, Kansas State University, Manhattan 66506. Corresponding author. E-mail: <dmt@gizmit.wheat.ksu.edu> (3) Presently at California Cereal Products, Oakland, CA 94607. Accepted February 13, 1996. Copyright 1996 by the American Association of Cereal Chemists, Inc. 

Wheat samples collected from various locations in Kansas were milled at 16% moisture content using a Buhler mill. Wheat bran, shorts, and flour from samples invaded with Fusarium graminearum and several noninvaded samples were analyzed for deoxynivalenol and zearalenone using thin-layer chromatography. The presence of deoxynivalenol and zearalenone on random samples was confirmed using gas chromatography. When present in milled fractions, the levels of deoxynivalenol and zearalenone were generally highest in the bran and lowest in the flour. Ten of 27 bran samples (37%) and six of 27 shorts samples (22%) contained deoxynivalenol levels that exceeded 1 ppm. Three of 27 samples (11%) yielded flour with deoxynivalenol levels that exceeded 1 ppm. Zearalenone levels exceeded 1 ppm in five of 27 bran samples (19%) and four of 27 shorts samples (15%). None of the flour fractions contained >1 ppm zearalenone.