doi:10.1094/CFW-59-4-0605 | VIEW ARTICLE

AACCI Report

AACCI Approved Methods Technical Committee Report: Collaborative Study on the Immunochemical Quantitation of Intact Gluten in Rice Flour and Rice-Based Products Using G12 Sandwich ELISA

ClydeDon,¹ElisabethHalbmayr-Jech,²AdrianRogers,³ and PeterKoehler<sup>4

1</sup>CDC Foodphysica, The Netherlands.²Romer Labs Division Holding GmbH, Tulln, Austria.³Romer Labs UK Ltd., Runcorn, Cheshire, U.K.⁴Deutsche Forschungsanstalt für Lebensmittelchemie, Leibniz Institut, Freising, Germany. Cereal Foods World 59(4):187-193.

The AACC International (AACCI) Protein and Enzyme Methods Technical Committee initiated a collaborative study of a method for gluten quantitation in selected foods using a G12 antibody sandwich ELISA system. The method has been approved as AACCI Approved Method 38-52.01. The new method has been validated for testing rice flour and rice-based products to verify that they conform to the defined Codex threshold of ≤20 mg of gluten/kg in products labeled “gluten-free.”