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Acrylamide is a well-known neurotoxin and carcinogen that is widely found in thermally processed foods. Due to the enormous diversity of acrylamide containing products on the market constant screening of products available to the consumers is critically important. Currently, methods for acrylamide detection require derivatization steps and skilled technicians which lead to high testing costs. Therefore, the purpose of this project was to develop a new method for acrylamide detection using NMR spectroscopy as a tool for providing reliable, high throughput method, with simple sample preparation. Acrylamide was extracted from various food products by d-chloroform, and distinct acrylamide peaks on 1H-NMR and 13C-NMR spectra were observed. Two-dimensional NMR confirmed coupling between acrylamide protons and their bonding to the appropriate carbons. Reliable quantification of acrylamide was confirmed by spiking bread with an acrylamide solution of known concentration and 100.3% recovery was obtained. Bread crust was tested, and value of 32 mg/kg was observed. Therefore our study proposes the new method of acrylamide detection, which can provide easy and reliable method for the large-scale acrylamide screening.