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Caroubin: A Gluten-like Protein Isolated from Carob Bean Germ

July 1998 Volume 75 Number 4
Pages 488 — 492
Pierre Feillet 1 , 2 and Thérèse Marie Roulland 1

Unité de Technologie des Céréales et des Agro-polymères, Institut National de la Recherche Agronomique, 2 place Viala, 34060 Montpellier cedex, France. Corresponding author. E-mail: feillet@ensam.inra.fr


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Accepted April 8, 1998.
ABSTRACT

In 1935, Bienenstock and coworkers claimed the presence of gluten-like material in the germ of the carob seed. The viscoelastic properties of the water-insoluble proteins isolated from carob germ, which we propose to call caroubin, have been confirmed by dynamic (G′ and G″) and static rheological measurements (texture profile analysis, viscoelastogram). Biochemical analyses showed important similarities (high glutamic acid content, size-exclusion HPLC profile, PAGE patterns of reduced and unreduced proteins) as well as large differences (high arginine and low cysteine and proline content of caroubin, carbohydrate composition) between caroubin and wheat gluten. Besides potential new industrial uses of carob seed, caroubin could be a valuable material to help us understand the physicochemical basis of the viscoelastic properties of plant protein complexes like wheat gluten.



This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. American Association of Cereal Chemists, Inc., 1998.