Cereals & Grains Association
Log In

03 Issues & Trends
Cereal Foods World, Vol. 64, No. 1
DOI: https://doi.org/10.1094/CFW-64-1-0007
Print To PDF
Rapid Methods for Detection of Pathogens in Dry Foods
Yuewei Hu
General Mills Inc., 9000 Plymouth Ave N, Minneapolis, MN 55427, U.S.A. Tel: +1.763.764.5426, E-mail: yuewei.hu@genmills.com

 

Abstract

Dry foods and dry food ingredients have been implicated with increased frequency as the cause of recent foodborne disease outbreaks. Traditional culture-based pathogen identification and quantification methods require approximately 3–5 days to obtain preliminary results. To improve method accuracy and efficiency, many rapid detection methods have been developed and commercialized. The speed and accuracy of these rapid tests allow stakeholders to make informed decisions that comply with food safety standards and minimize the risk of potential foodborne disease outbreaks and/or product recalls. This article provides an overview of a variety of commercially available rapid methods for detection of pathogens in dry foods and dry food ingredients, including both antibody-based and nucleic acid-based methods.




Trying to reach content?

View Full Article

if you don't have access, become a member

References

  1.  Alahi, M. E. E., and Mukhopadhyay, S. C. Detection methodologies for pathogen and toxins: A review. Sensors 17:1, 2017.
  2. Beuchat, L. R., Komitopoulou, E., Beckers, H., and Betts, R. O. Y. P. Low-water activity foods: Increased concern as vehicles of foodborne pathogens. J. Food Prot. 76:150, 2013.
  3. Bicart-See, A., Rottman, M., Cartwright, M., Seiler, B., Gamini, N., et al. Rapid isolation of Staphylococcus aureus pathogens from infected clinical samples using magnetic beads coated with Fc-mannose binding lectin. PLoS One. DOI: https://doi.org/10.1371/journal.pone.0156287. 2016.
  4. Centers for Disease Control and Prevention. List of selected multistate foodborne outbreak investigations. Published online at www.cdc.gov/foodsafety/outbreaks/multistate-outbreaks/outbreaks-list.html. CDC, Atlanta, GA, 2018.
  5. Cho, I.-H., Bhandari, P., Patel, P., and Irudayaraj, J. Membrane filter-assisted surface enhanced raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef. Biosens. Bioelectron. 64:171, 2015.
  6. Dwivedi, H. P., and Jaykus, L. A. Detection of pathogens in foods: The current state-of-the-art and future directions. Crit. Rev. Microbiol. 37:40, 2011.
  7. Farakos, M. S., and Frank, J. F. The microbiological safety of low water activity foods and spices. Food Microbiol. Food Saf. DOI: 10.1007/978-1-4939-2062-4_2. 2014.
  8. Keith, M. Evaluation of an automated enzyme-linked fluorescent immunoassay system for the detection of salmonellae in foods. J. Food Prot. 60:682, 1997.
  9. Lee, K. M., Runyon, M., Herrman, T. J., Phillips, R., and Hsieh, J. Review of Salmonella detection and identification methods: Aspects of rapid emergency response and food safety. Food Control 47:264, 2015.
  10. Padmapriya, P., and Banada, A. K. B. Antibodies and Immunoassays for Detection of Bacterial Pathogens. Springer, New York, NY, 2008.
  11. Shim, W. B., Choi, J. G., Kim, J. Y., Yang, Z. Y., Lee, K. H., et al. Enhanced rapidity for qualitative detection of Listeria monocytogenes using an enzyme-linked immunosorbent assay and immunochromatography strip test combined with immunomagnetic bead separation. J. Food Prot. 71:781, 2008.
  12. Zitz, U., Zunabovic, M., Domig, K. J., Wilrich, P. T., and Kneifel, W. Reduced detectability of Listeria monocytogenes in the presence of Listeria innocua. J. Food Prot. 74:1282, 2011.