Cereal Chem 43:237 - 243.  |  VIEW ARTICLE

The Application of Mathews' Formula in Enzymatic Starch Conversions.

T. Cayle and F. Viebrock. Copyright 1966 by the American Association of Cereal Chemists, Inc. 

Mathews' formula, PT/D, wherein P refers to polarization, T, Lane-Eynon titration, and D, dilution, was originally developed to determine the ratio of fructose to total reducing sugar in glucose-fructose mixtures. The concept has now been applied to the monitoring of the hydrolysis products of the enzymatic conversion of starch with alpha-amylase and amyloglucosidase. In this case the primary product is glucose - small quantities of maltose, isomaltose, and panose being the usual contaminants, depending on the purity of the saccharifying enzyme and its biological source. The use of Mathews' formula allows for the rapid estimation of nonglucose components and, conversely, glucose, with a polarimeter reading and a titration the only data required.