Cereal Chem 43:546 - 554.  |  VIEW ARTICLE

Partial Purification of Proteases from Germinated Barley.

W. C. Burger, N. Prentice, J. Kastenschmidt, and J. D. Huddle. Copyright 1966 by the American Association of Cereal Chemists, Inc. 

Proteases from germinated barley have been separated by treatment with carboxymethyl cellulose and by filtration of products therefrom through a dextran gel. These consist of (a) an acidic peptidase which hydrolyzes alpha-N-benzoyl-DL-arginine-p-nitroanilide (BAPA-ase), together with an acidic peptidase which hydrolyzes alpha-N-benzoyl-L-arginine ethylester (BAEE-ase) and an acidic proteinase; (b) two separated acidic proteinases; (c) a neutral BAEE-ase together with a neutral proteinase; and (d) a separated neutral proteinase.