Cereal Chem 52:714 - 718.  |  VIEW ARTICLE
Sulfanilamide-Azogluten: A Chromophoric Gluten Derivative for Colorimetrically Estimating Protease Activity on Gluten.

J. W. Finley. Copyright 1975 by the American Association of Cereal Chemists, Inc. 

A chromophoric protein derivative has been formed by reacting red azo dye sulfanilamide with gluten. The sulfanilamide-azogluten is acted on by various proteases and offers a convenient quantitative method for predicting activity of various proteases on gluten. The generated soluble dye-bound peptides are easily separated and can be measured with a simple spectrometer.