Cereal Chem 54:905 - 917.  |  VIEW ARTICLE
Automated Liquid Chromatographic System for Analysis of Carbohydrate Mixtures.

H. D. Scobell, K. M. Brobst, and E. M. Steele. Copyright 1977 by the American Association of Cereal Chemists, Inc. 

An automated liquid chromatographic (LC) system is described for quantitative analysis of carbohydrate mixtures. Dextrose, fructose, and related saccharides of commercial sweeteners are separated on cation- exchange resins with water as the eluent. The column systems described give rapid, high-resolution separations on the stable baseline---features which allow accurate and precise quantitation. All the carbohydrate components of a sweetener elute from the columns, permitting quantitation on a simple area normalization basis. No supporting analyses are required. Analysis time ranges from 5 to 30 min. The instrumentation described includes a custom-built automatic sample injector/system controller, eoupled with a conventional LC and a computing integrator.