Cereal Chem 54:333 - 344.  |  VIEW ARTICLE
Chromatographic Fractionation and Composition of the Components of the Salt-Soluble Proteins from Hiproly (CI 3947) and Hiproly Normal (CI 4362) Barleys.

A. M. El-Negoumy, C. W. Newman, and B. R. Moss. Copyright 1977 by the American Association of Cereal Chemists, Inc. 

Fractionation of the protein complex in Hiproly (CI 3947) and Hiproly Normal (CI 4362) barleys showed that the salt-soluble protein fraction accounted for 41.8 and 43.5% of the total lysine, respectively. Salt- soluble and glutelin fractions together accounted for 78.7% of the total lysine in Hiproly and 80.6% in Hiproly Normal. Salt-soluble protein fractions from these two barleys contained identical protein components, as indicated by comparison of the electrophoretic mobilities of components of chromatographic fractions. One chromotographic fraction accounted for 50.3% of the lysine in the salt- soluble proteins of Hiproly and 43.5% in Hiproly Normal. The first three chromatographic fractions from the salt-soluble protein of Hiproly contained 52% more lysine than did comparable fractions in Hiproly Normal. These fractions contained higher percentages of aspartic acid, lysine, and valine and lower percentages of tyrosine, glutamic acid, and cystine/2 in Hiproly than in Hiproly Normal.