Cereal Chem 54:588 - 596.  |  VIEW ARTICLE
Studies of Glutenin. IX. Subunit Composition by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis at pH 7.3 and 8.9.

K. Khan and W. Bushuk. Copyright 1977 by the American Association of Cereal Chemists, Inc. 

Glutenin subunit compositions of three Canadian hard red spring wheat cultivars, Prelude, Rescue, and Thatcher, their extracted AABB tetraploid lines, and several other wheats were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) at pH 7.3 and pH 8.9. Results from these experiments differed from published results; five high-molecular weight subunits were found for the hexaploid wheats: 134,000 (1), 132,000 (2), 110,000 (3), 98,000 (4), 90,000 (5). Extracted tetraploids Tetraprelude and Tetrathatcher lacked subunits 1 and 5, whereas Tetrarescue lacked subunits 2 and 5. The natural AABB tetraploid, Stewart 63, a durum wheat cultivar, lacked subunits 1, 2, and 5. The variety Chinese Spring, a hexaploid cultivar used extensively in cytogenetic studies, lacked subunit 2 but contained the other four high-molecular weight subunits. The first two high-molecular weight subunits were better resolved at pH 7.3 for most of the hexaploid wheats examined, whereas subunits 4 and 5 were better resolved at pH 8.9. For complete resolution of the high-molecular weight subunits, it is necessary to use a combination of SDS-PAGE methods.