Cereal Chem 62:227-229   |  VIEW ARTICLE
Gliadin High-Performance Liquid Chromatography and Polyacrylamide Gel Electrophoresis Patterns of Wheats Grown with Fertilizer Treatments in the United States and Australia on Sulfur- Deficient Soils.

G. L. Lookhart and Y. Pomeranz. Copyright 1985 by the American Association of Cereal Chemists, Inc. 

Gliadin protein patterns were determined by polyacrylamide gel electrophoresis (PAGE) and high- performance liquid chromatography (HPLC) for wheats grown with various fertilizer treatments on sulfur- deficient soils in Washington state (USA) and in Australia. The patterns of those samples, grown on sulfur- deficient soil to which nitrogen was added, exhibited higher relative intensities of their 73 relative mobility band than the relative intensity of that band in corresponding samples from unfertilized soil. The samples from Australia, which differed in their response to a semiquantitative sulfur deficiency test, also differed in PAGE and HPLC patterns. Wheat grown on sulfur-deficient soil, which had been fertilized with sulfur, showed more intense high mobility PAGE bands and less intense low mobility bands than wheat grown on unfertilized sulfur-deficient soil. Similarly, the peaks eluting in the 20-25 min range from the HPLC column were smaller and those eluting in the 43-55 min range were larger from the Australian wheat grown on sulfur-fertilized than on unfertilized soil.