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Inhibition of intestinal glucosidase by feruloylated arabinoxylan oligosaccharides from corn bran and wheat aleurone
L. MALUNGA (1), P. Eck (1), T. Beta (1). (1) University of Manitoba, Winnipeg, MB, Canada
The effect of feruloylated arabinoxylan oligosaccharides (FOS) on mammalian α-glucosidase was investigated using human caco2 cells and rat intestinal acetone powder. The isolated FOS from wheat aleurone and corn bran were identified to have degree of polymerisation (DP) of 4 and 1, respectively, by HPLC-MS. Both FOS extracts were effective inhibitors of sucrase and maltase functions of the α-glucosidase. The IC50 for FOS extracts on caco2 cells and rat intestinal α-glucosidase was 1.03 – 1.65 mg/mL and 2.6 – 6.5 mg/mL, respectively. The inhibitory effect of FOS was dependent on their ferulic acid (FA) content (R<sup>2</sup> = 0.95). The IC50 of free FA on caco2 cells and rat intestinal α-glucosidase was 0.09 and 0.22 mg/mL, respectively. At equal ferulic acid concentration (0.04 mg/mL), FA, FOS from corn and wheat inhibited sucrase activity by 18.70 ± 5.25, 23.28 ± 4.51% and 19.45 ± 4.85% respectively. Similarly, maltase activity was decreased by 38.85 ± 3.01, 24.45 ± 3.42 and 17.11 ± 4.07 %, respectively. Our results based on limited samples suggest that degree of polymerisation does not affect the potency of FOS on α-glucosidase activity. Thus, feruloylated arabinoxylan oligosaccharides has potential for use in diabetes management.
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